What is OneTaq?
OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments. Unique hot start formulation eliminates non-specific amplification and does not require a separate activation step. Robust yield with minimal optimization.
What is the role of the DNA polymerase in the OneTaq protocol?
Overview. The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification. Taq DNA Polymerase is an enzyme widely used in PCR. OneTaq DNA Polymerase allows for greater amplification sensitivity across a wide variety of amplicons regardless of GC content.
What is Taq1 Polymerase?
A heat stable DNA-DIRECTED DNA POLYMERASE from the bacteria Thermus aquaticus. It is widely used for the amplification of genes through the process of POLYMERASE CHAIN REACTION.
Is Vent polymerase thermostable?
Vent® DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA Polymerase is 5-15-fold higher than that observed for Taq DNA Polymerase (1,2).
Is Pfu polymerase thermostable?
Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus.
What is Vent polymerase used for in PCR?
Vent polymerase is a thermostable archean DNA polymerase used for the polymerase chain reaction. It was isolated from the thermophile Thermococcus litoralis….Vent DNA polymerase.
| DNA polymerase | |
|---|---|
| Identifiers | |
| Organism | Thermococcus litorali |
| Symbol | pol |
| UniProt | P30317 |
Which polymerase is thermostable?
Taq polymerase
Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976.
Why do you prefer thermostable DNA polymerase in PCR?
A thermostable DNA polymerase helps in the possessing of proofreading activity is desirable for high range of amplification such as when amplifying large segments of DNA which are found at low copy-number and each copy may be a sequence variant concerning the others when amplifying genes where the exact sequence is …
What is GC clamp?
Simply, a GC clamp is the presence of a guanine (G) or cytosine (C) base in the last 5 bases (the 3′ end) of a PCR primer. Having the presence of a GC clamp in a PCR primer can help to improve the specificity of primer binding to the complementary sequence.
What are the 3 cycles of PCR?
PCR is based on three simple steps required for any DNA synthesis reaction: (1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the primers.
What is the difference between Taq and Pfu polymerase?
The main difference between Pfu and alternative enzymes is that Pfu has superior thermostability and proof-reading properties. Unlike Taq DNA polymerase, Pfu DNA polymerase also possesses 3′->5′ exonuclease proofreading activity, resulting in PCR fragments with fewer errors than Taq-generated PCR inserts.
What is Taq and Pfu polymerase?
Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity.
Why is thermostable polymerase needed for PCR?
In the polymerase chain reaction (PCR) a thermostable DNA polymerase and two specific oligonucleotide primers are used to produce multiple copies of specific nucleic acid regions quickly and exponentially (Fig. 6.4).