What does BAPTA stand for?
BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid) is a calcium-specific aminopolycarboxylic acid. The presence of four carboxylic acid functional groups makes possible the binding of two calcium ions.
How does Bapta work?
BAPTA, AM is a cell-permeant chelator, which is a highly selective for Ca2+ over Mg2+ , and it can be used to control the level of intracellular Ca2+. BAPTA is more selective for Ca2+ than EDTA and EGTA, and its metal binding is also less sensitive to pH. For Research Use Only. Not for use in diagnostic procedures.
What is the role of EGTA?
EGTA is used as a compound in elution buffer in the protein purification technique known as tandem affinity purification, in which recombinant fusion proteins are bound to calmodulin beads and eluted out by adding EGTA. EGTA is often employed in dentistry and endodontics for the removal of the smear layer.
What is used to chelate calcium?
The chelating agent calcium ethylenediaminetetraacetic acid (EDTA) (calcium disodium salt of EDTA) is indicated for acute exposure if administered shortly after cadmium exposure before new metallothionein is synthesized.
What does Bapta target?
BAPTA-AM is a cell-permeable derivative which is widely used as an intracellular calcium sponge. Induces inactivation of protein kinase C….Product Details.
| Alternative Name: | 1,2-bis-(o-Aminophenoxy)-ethane-N,N,N’,N’-tetraacetic acid, tetraacetoxymethyl ester |
|---|---|
| CAS: | 139890-68-9 |
| Purity: | ≥90% (HPLC) |
What is the difference between EDTA and EGTA?
EDTA has a higher affinity for Mg2+ ions compared to EGTA. Ethylene glycol tetraacetic acid (EGTA) is also a chelating agent. Compared to EDTA, it has a higher affinity for calcium ions but a lower affinity for magnesium ions. Similar to EDTA, EGTA can be used as a buffer to resemble the pH of a living cell.
How do you dilute EGTA?
For a 100 mM EGTA stock solution, add 3.8 g to about 20 ml of distilled H2O and bring to pH 11 with NaOH; then bring to pH 8.0 with HCl and add H2O to a final volume of 100 ml. Dissolve and adjust to 150 ml with ultrapure H2O. Filter-sterilize and store at room temperature.
How many chelates are in CA EDTA?
Ethylenediamine tetraacetic acid (EDTA), which has many coordinated atoms, forms a very stable chelate between one molecule of EDTA and metal ion. Chelating reagents are utilized for chelate titration, isolation, and separation of metal ions….Ca(II)-EDTA.
| Item # | Unit Size |
|---|---|
| E008-10 | 50 g |
How do you dissolve Bapta am?
BAPTA AM is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, BAPTA AM should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. BAPTA AM has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method.
Which is the commonly used chelator?
Calcium disodium ethylenediamine tetraacetic acid (CaNa2EDTA) is the most commonly used chelating agent. It is a derivative of ethylenediamine tetraacetic acid (EDTA); a synthetic polyamino-polycarboxylic acid and since 1950s has been one of the mainstays for the treatment of childhood lead poisoning [12].
Is EGTA a chelating agent?
EGTA is a chelating agent that is related to the better known EDTA, but with a much higher affinity for Ca2+than for Ca2+ ions. The pKa for binding of calcium ions by tetrabasic EGTA is 11.00, but the protonated forms do not significantly contribute to binding, so at pH 7, the apparent pKa becomes 6.91.
What is a BAPTA chelator?
BAPTA is a non-permeable, selective extracellular calcium chelator, with 105-fold greater affinity for Ca 2+ than Mg 2+. BAPTA is a valuable tool to study the role of calcium in cell signaling. For research use only.
What is the mechanism of action of BAPTA AM?
BAPTA-AM is a well-known membrane permeable Ca2+ chelator. BAPTA-AM inhibits hERG channels, hKv1.3 and hKv1.5 channels in HEK 293 cells with IC50 s of 1.3 μM, 1.45 μM and 1.23 μM, respectively [1] .
What is BAPTA-AM?
BAPTA-AM est un chélateur Ca 2+ perméable à la membrane bien connu. BAPTA-AM inhibe les canaux hERG, hKv1.3 et hKv1.5 dans les cellules HEK 293 avec IC 50 de 1,3 μM, 1,45 μM et 1,23 μM, respectivement. BAPTA-AM ist ein bekannter membrandurchlässiger Ca 2+ chelator.
Does Nisoldipine suppress the APD-lengthening effect of BAPTA-AM?
The APD-lengthening effect of BAPTA-AM was strongly suppressed in the presence of nisoldipine, and enhanced in the presence of BAY K8644, suggesting that a shift in the [Ca 2+] i -dependent inactivation of L-type Ca 2+ current may be an important underlying mechanism.