How do I order TaqMan assays?
Click “TaqMan® Gene Expression Assays” under the “Assays & Arrays” section.
- STEp 1: gO TO TAqMAn gEnE ExpRESSIOn ASSAyS fROM ApplIED BIOSySTEMS HOME pAgE (WWW.ApplIEDBIOSySTEMS.COM)
- STEp 2: SEARCHIng fOR ASSAyS.
- STEp 3: RESulTS.
- STEp 4: ASSAy DETAIlS pAgE.
- STEp 6: SElECT ASSAyS.
- STEp 7: ORDERIng.
- STEp 5: lOg In.
Why is TaqMan used?
TaqMan-based detection Uses Applied Biosystems™ SYBR™ Green dye (a dsDNA binding dye) to detect PCR product as it accumulates during PCR. Uses a fluorogenic probe specific to target gene to detect target as it accumulates during PCR. *Depends on template quality and primer/design optimization.
What is Vic MGB PL?
Recommendations. The Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™ ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays.
Can TaqMan assay be used to detect viral infection?
TaqMan PCR is widely implemented as a detection and quantitation method for viruses isolated from a variety of sample types.
When was TaqMan invented?
The method was first reported in 1991 by researcher Kary Mullis at Cetus Corporation, and the technology was subsequently developed by Hoffmann-La Roche for diagnostic assays and by Applied Biosystems (now part of Thermo Fisher Scientific) for research applications.
What is Tamra quencher?
TAMRA dye is an effective quencher for fluorophores with emission maxima less than 560 nm. Dyes with longer wavelength emissions will not be effectively quenched by TAMRA. In addition, TAMRA has its own fluorescence which complicates data analysis due to crosstalk between the channels.
What is a TaqMan gene expression assay?
TaqMan® Gene Expression Assays are based on 5′ nuclease chemistry, which uses a fluorogenic probe to enable the detection of a specific PCR product as it accumulates during PCR. A TaqMan probe with a FAM™ or VIC™ dye label on the 5′ end, and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3′ end
How do I order custom TaqMan® assays?
Use the Custom TaqMan®Assay Design Tool to order: • Custom TaqMan®SNP Genotyping Assays— Custom assays for performing genotyping studies with singlenucleotide polymorphisms (SNPs), multiple nucleotide polymorphisms (MNPs), or insertions/deletions (indels). For more information, go to thermofisher.com/taqmansnpdesign.
What is TaqMan fast real-time PCR?
TaqMan Fast real-time PCR The ligation product serves as a DNA template for real-time PCR using a TaqMan Protein Assay. The mechanism of action is the same as that of the gene expression assay, using unlabeled primers and a TaqMan probe with a fluorescent label on the 5´ end and a nonfluorescent quencher (NFQ) on the 3´ end.
What is a TaqMan test used for?
TaqMan Mutation Detection Assays are powered by allele-specific castPCR technology to detect and measure somatic mutations in genes. They are compatible with different sample types, such as cell lines, FFPE tissue samples, and fresh-frozen tissue samples.