What is selectable marker PPT?
Selectable marker Selectable marker is required for maintenance of plasmid in the cell. It is a genes that confers resistance to particular antibiotics. Genes that make cells resistance to ampicillin, neomycin or chloramphenicol.
What are selectable marker genes used for?
Selectable Markers Selectable marker genes are conditionally dominant genes that confer an ability to grow in the presence of applied selective agents that are normally toxic to plant cells or inhibitory to plant growth, such as antibiotics and herbicides.
Which gene is a selectable marker?
Selectable markers are often antibiotic resistance genes (An antibiotic resistance marker is a gene that produces a protein that provides cells expressing this protein with resistance to an antibiotic.).
What is genes PPT?
The gene is the unit of genetic information that controls a specific aspect of the phenotype. The gene is the unit of genetic information that specifies the synthesis of one polypeptide. 6. genes are the fractions or part of DNA molecule which regarded as the genetic material.
What is selectable marker and example?
Selectable markers are the genes present in the cloning vectors that help in eliminating the non-transformants. E.g. antibiotics resistance genes, herbicide resistance genes, etc.
What is the role of selective markers in vectors?
Selectable markers are often antibiotic resistance genes. They help in artificial selection. They help in identifying transformants and selectively allowing their growth whereas eliminating non-transformants in cloning vector.
What is chromosome PPT?
Chromosome means: chroma – colour; some – body) A chromosome is a thread-like self-replicating genetic structure containing organized DNA molecule package found in the nucleus of the cell.
How do selectable markers work?
Hint:A selectable marker helps to identify and delete non-transformants and allows processors to expand selectively. A selectable marker is a gene inserted into a cell, in particular a bacterium or a cultured cell, which confers a trait appropriate for artificial selection.
What is the importance of selectable markers in recombinant DNA technology?
Selectable markers are essential to identify and eliminate non-transformants(no recombinant DNA), and selectively permitting the growth of the transformants (host cells bearing recombinant DNA).
What is cell cycle PPT?
CELL CYCLE A cell cycle is a series of events that a cell passes through from the time until it reproduces its replica. It is the growth and division of single cell into daughter cells and duplication (replication). In prokaryotic cells, the cell cycle occurs via a process termed binary fission.
What is DNA Slideshare?
DNA DNA or deoxyribonucleic acid is a genetic material that transfer the genetic information from one organism to their off spring. Located in nucleus and mitochondria The information in DNA is stored as code (made up of A,G,C,T). 99% of base are same . The order of bases determines the individuality.
What is the importance of selectable marker?
Why is it important to have selectable markers?
What is a plasmid PPT?
Plasmids are circular deoxyribonucleic acid (DNA) molecules that replicate independently of the bacterial chromosome. They are not essential for the bacterium but may confer a selective advantage.
What are selectable marker genes?
• Selectable marker genes enable the transformed cells to survive on media containing selection agent which causes death of non-transformed cells. • Examples – antibiotic, antimetabolite, and herbicide resistance genes etc. 2. Selectable marker genes: Selectable marker gene Near target gene Detectable and distinguishable Inherited along with target
What is the most widely used selectable marker?
The most widely used selectable marker is npt II gene encoding the enzyme neomycin phosphotransferase II (NPT II). This marker gene confers resistance to the antibiotic kanamycin. The trans-formants and the plants derived from them can be checked by applying kanamycin solution and the resistant progeny can be selected.
How do you clone a selectable marker gene?
Cloning of selectable markers between transposable elements: A selectable marker gene can be cloned between plant transposable elements (Ds elements) and then inserted. The selectable marker is planked by the sequences that increase the intra-chromosomal recombination. This results in the excision of the marker gene.
What are the antibiotic resistance selectable marker genes?
Some of the antibiotic resistance selectable marker genes are briefly described. The most widely used selectable marker is npt II gene encoding the enzyme neomycin phosphotransferase II (NPT II). This marker gene confers resistance to the antibiotic kanamycin.