What does Bapta am do?
BAPTA, AM is a cell-permeant chelator, which is a highly selective for Ca2+ over Mg2+ , and it can be used to control the level of intracellular Ca2+. BAPTA is more selective for Ca2+ than EDTA and EGTA, and its metal binding is also less sensitive to pH. For Research Use Only. Not for use in diagnostic procedures.
What does Bapta stand for?
BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid) is a calcium-specific aminopolycarboxylic acid. The presence of four carboxylic acid functional groups makes possible the binding of two calcium ions.
How do you dissolve a Bapta?
BAPTA is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, BAPTA should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. BAPTA has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method.
What does BAPTA do to calcium?
Intracellular calcium chelator BAPTA protects cells against toxic calcium overload but also alters physiological calcium responses. Cell Calcium.
What is calcium chelator?
Chelators are compounds that bind to metal ions forming a complex and some of them are also fluorescent. If the complex has fluorescence properties different from those of the free chelator, it can be used as an ion indicator.
Is Bapta cell permeable?
BAPTA-AM is a well-known membrane permeable Ca(2+) chelator.
What are calcium chelators?
What does EDTA mean in a blood test?
What is EDTA? EDTA, short for ethylenediaminetetraacetic acid, is a fairly standard part of blood collection. It is used not only as a way to keep blood from clumping together, but also to treat extreme cases of lead poisoning in a process called “chelation therapy.”
Where can I find the terms and conditions for the Abcam trial?
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial. Which is concentration of antibody (1µg/ml) used to detect PAI-1 in human plasma?
What is synthetic peptide antibody?
Synthetic peptide corresponding to Human PAI1 aa 100-200 conjugated to keyhole limpet haemocyanin. This antibody gave a positive signal in the following Lysates: WB: HUVEC whole cell lysate and Human Aortic Endothelial Cell Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney. ICC/IF: HeLa cell line.
What is the concentration of pai1 for heat mediated antigen retrieval?
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human PAI1 peptide (ab66704).
What is the relationship between octmab™ and pai1 expression?
Increase in PAI1 expression correlates with increased concentration of OcTMAB™, as described in literature.