What is MEF in biology?

Table of Contents

Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line.

What are MEF cells used for?

MEF cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

Why do we use mouse embryonic fibroblasts?

Abstract. Mouse embryonic fibroblasts (MEFs) are commonly collected as a means to maintain the culture and growth of embryonic stem cells (ESCs). However, their utility can extend well beyond their use exclusively in ESC culture.

What are MEF feeder cells?

Embryonic Fibroblast (MEF) feeder cells are used for the maintenance of mouse or human ES cells in the undifferentiated state. The cells must be mitotically inactivated prior to the addition of ES cells, such as treatment with mitomycin C (2-4 hr, 10 µg/mL).

How do you isolate a MEF cell?

Making MEFs

Place freshly harvested embryos in 10cm cell culture dish.
Cover embryos in PBSA and remove placental and other maternal tissues.
Cut away top of head (eye and above) and eviscerate removing all innards.
Save head for DNA isolation for genotyping (or yolk sac)
Place embryo body in separate 10cm plate.

Where does induced pluripotent stem cells come from?

Induced pluripotent stem (iPS) cells, are a type of pluripotent stem cell derived from adult somatic cells that have been genetically reprogrammed to an embryonic stem (ES) cell-like state through the forced expression of genes and factors important for maintaining the defining properties of ES cells.

How do you create a MEF?

What are fibroblast cells?

A fibroblast is a type of cell that contributes to the formation of connective tissue, a fibrous cellular material that supports and connects other tissues or organs in the body. Fibroblasts secrete collagen proteins that help maintain the structural framework of tissues.

How do you use embryonic fibroblasts in culture mouse?

Culture and passage of mouse embryonic fibroblasts. To obtain cells for culture, carefully place a 5 ml pipette through the supernatant and pipette up the pellet along with 0.5–1 ml of the supernatant (Figure 2H, 2I), and place in a 100-mm dish containing 10 ml complete culture medium for each embryo.

What is in MEF conditioned media?

MEF conditioned media represents an efficient alternative to MEF feeder cells and is one of the mostly widely used feeder-free systems for embryonic stem cell culture. MEF Conditioned Media includes the soluble factors required to support stem cell growth and pluripotency.

How do you transfect a MEF cell?

7A) Replate cells into 10 cm plates. Remove medium from each well, add 1 mL 0.05% trypsin/EDTA, let sit at room temperature for ~ 5 min (time not critical), then add 2 mL DMEM with 10% FBS. Transfer all 3 mL to 10 cm dish containing 10 mL of same medium. Return to incubator and let cells grow and recover overnight.

What is the difference between embryonic and induced pluripotent stem cells?

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of preimplantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc).

What is an induced pluripotent stem cell and how is it created?

iPSC are derived from skin or blood cells that have been reprogrammed back into an embryonic-like pluripotent state that enables the development of an unlimited source of any type of human cell needed for therapeutic purposes.

What is the full form of MEF?

Multipurpose Empanelment Form (MEF)

What does MEF stand for in networking?

Metro Ethernet Forum
The MEF, as the defining body for Carrier Ethernet, is a global industry alliance comprising more than 220 organizations including telecommunications service providers, cable MSOs, network equipment/software manufacturers, semiconductors vendors and testing organizations.

What is fibroblast and its function?

What is the role of fibroblast?

Fibroblasts are critical in supporting normal wound healing, involved in key processes such as breaking down the fibrin clot, creating new extra cellular matrix (ECM) and collagen structures to support the other cells associated with effective wound healing, as well as contracting the wound.

How do you get MEF cells?

What is human fibroblast conditioned media?

Human Fibroblast Conditioned Media (HFCM) essentially means a “Growth Factor Cocktail” for your skin. We have written more about the most common GF used in skincare, the Epidermal Growth Factor here, so if you are new to the topic head over there to catch up.

How do you isolate MEFs?

Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast derived from the mouse embryo. MEFs have a typical characterization of spindle shape in vitro which is a typical feature of fibroblasts. As a kind of primary cell, MEFs will senescence and finally die off after limited transmission.

What is MEF (mouse embryonic fibroblast)?

Spindle cells surrounding the stem cell colony are MEFs. Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line. After several transmission, MEFs will senesce and finally die off.

What are MEF spindle cells?

What are MEF feeder cells used for?

Most commonly used MEF feeder cell line used to support mouse and human embryonic stem cells (ES Cells) in culture. Neomycin resistant MEF cells isolated from FVB mouse embryos at day 13. Commonly used in genetic selection experiments using neomycin as selection agent.