Does SacI have star activity?
Time-Saver™ qualified for digestion in 5-15 minutes. Reduced star activity. Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Is SacI a restriction enzyme?
SacI is a restriction enzyme isolated from the bacterium Streptomyces achromogenes.
What is the full form of Sacii?
ASCII: American Standard Code for Information Interchange The ASCII codes represent computers and other communication devices that use text. It was the most common computer encoding on the World Wide Web until December 2007, after that it surpassed by UTF-8 which uses ASCII as a subset.
Is Hindiii methylation sensitive?
Not sensitive to dam, dcm or mammalian CpG methylation.
What are restriction enzymes?
A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.
Are isoschizomers interchangeable?
Isoschizomers are interchangeable, and neoschizomers are not, because they cut differently even if they recognize the same sequence.
What is NEBcutter used for?
NEBcutter — A restriction analysis tool Find restriction digestion map of a DNA sequence. Highlights: This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E. coli genetic code and the sites for all Type II restriction enzymes that cut the sequence just once.
What is a restriction buffer?
Reaction buffers for use in experiments involving restriction enzymes, such as restriction digestions. Different buffer solutions create optimal reaction conditions for specific restriction enzymes, or facilitate multiple enzymatic reactions.
What are the five restriction enzymes?
Naturally occurring restriction endonucleases are categorized into five groups (Types I, II, III, IV, and V) based on their composition and enzyme cofactor requirements, the nature of their target sequence, and the position of their DNA cleavage site relative to the target sequence.