What is the excitation wavelength for Alexa 488?
Alexa Fluor 488 is a fluorescent compound with an excitation peak at 499 nm and an emission peak at 520 nm. It can be excited using a 488 nm laser paired with a 530/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSAria™ Fusion.
How does Alexa Fluor 488 work?
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
What Colour is Alexa Fluor 488?
green-
Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line.
Is Alexa 488 FITC?
Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC.
What is DyLight 488 and why is it essential?
DyLight® 488 is a green fluorophore commonly used in applications such as protein labeling, immunolabeling, fluorescence microscopy, and enzyme immunoassays. The dye has an absorption wavelength that peaks around 493 nm, and an emission maximum around 518 nm.
What is the excitation and emission of PE?
PE (phycoerythrin) is a fluorescent compound with an excitation peak at 566 nm and an emission peak at 574 nm. It can be excited using a 561 nm laser paired with a 585/20 nm bandpass filter, a configuration that can be found, for example, in the BD Special Order LSRFortessa™ Cell Analyzer.
Does PE bleed into FITC?
where FITC bleeds into the PE channel and PE bleeds back into FITC. To correct for spectral overlap during multicolor flow cytometry experiments, color compensation must be performed. The goal of color compensation is to correctly quantify each dye with which a particular cell is labeled.
Can I use PE and PE Cy7 together?
In my experience, it is not advisable to use APC/Cy7 and PE/Cy7 together due to heavy cross-beam contamination as their emission max are exactly same. However, they can be used together if you are using machines like the Cytek Aurora.
Is R PE the same as PE?
* PE is the same as R-phycoerythrin. APC, allophycocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin; PerCP, peridinin chlorophyll protein.
What happens when UV light excites a fluorochrome?
During fluorescence imaging, the item being inspected absorbs a shorter wavelength of light (usually ultraviolet (UV) light) that excites a specific fluorophore, causing it to release photons that fluoresce and emit light at a longer wavelength. In industrial applications this fluorescence is most often a blue color.
Is Alexa Fluor 488 same as FITC?
Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC. However, Alexa Flour 488 display higher photostability and is less susceptible to self-quenching.
What is fluorescence excitation and emission?
A fluorescence emission spectrum is when the excitation wavelength is fixed and the emission wavelength is scanned to get a plot of intensity vs. emission wavelength. A fluorescence excitation spectrum is when the emission wavelength is fixed and the excitation monochromator wavelength is scanned.
Is PE-Cy7 bright?
PE-Cy7 conjugated reagents are as bright as PE conjugates. However, PE-Cy7 is highly sensitive to photo-induced degradation resulting in loss of fluorescence. Care should be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative.
What is the emission maximum of Alexa Fluor 488?
Alexa 488 absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell and tissue staining of multiple proteins with Alexa Fluor ® 594 and Alexa Fluor ® 647 conjugated secondary antibodies. Emission max.: Emission max.:
What can Alexa Fluor 488 dye conjugate to?
We offer Alexa Fluor 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
What is the peak wavelength of Fluor 488 antibodies?
Alexa Fluor® 488 – conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm.
What is the difference between Fluor 488 and FITC?
Alexa Fluor ® 488 conjugated antibodies advantages compared to Fluorescein isothiocyanate (FITC) make it the most used green fluorophore for fluorescent cell staining. Comparable spectral properties and quantum yield. Brighter and more photostable when conjugated to antibodies.