What is a migration assay?
The transwell migration assay is a commonly used test to study the migratory response of endothelial cells to angiogenic inducers or inhibitors. This assay is also known as the Boyden or modified Boyden chamber assay.
What is the purpose of a migration assay?
The transwell cell migration assay measures the chemotactic capability of cells toward a chemo-attractant. The transwell cell invasion assay, however, measures both cell chemotaxis and the invasion of cells through extracellular matrix, a process that is commonly found in cancer metastasis or embryonic development.
How do you scratch assay?
Scratch assay on non-transfected cells Scrape the cell monolayer in a straight line to create a “scratch” with a p200 pipet tip. Remove the debris and smooth the edge of the scratch by washing the cells once with 1 ml of the growth medium and then replace with 5 ml of medium specific for the in vitro scratch assay.
How is cell motility measured?
The simplest approach to monitoring cell migration is the so-called “scratch” assay. A pipette tip or other sharp object is used to gouge a scratch or “wound” in a confluent cell monolayer. Then a microscope is used to observe cells filling in or “repairing” the wound.
What is the purpose of scratch assay?
The scratch-wound assay is a simple, reproducible assay commonly used to measure basic cell migration parameters such as speed, persistence, and polarity. Cells are grown to confluence and a thin “wound” introduced by scratching with a pipette tip. Cells at the wound edge polarise and migrate into the wound space.
What is invasion assay?
These 96-well microplate-based assays are designed to accelerate the screening process for compounds that influence chemotaxis. The Cell Migration Assay measures the number of cells traversing a porous membrane, while the Cell Invasion Assays monitor cell movement through extracellular matrices.
How is scratch assay measured?
Data analysis – scratch closure The first method measures the change in the wound width (nanometres) over time. This width is the average distance between the two margins of the scratch. The second method calculates the change in wound area over time as a percentage of wound closure.