What is the molecular weight of proteinase K?
28,900 daltons
The molecular weight of Proteinase K is 28,900 daltons (28.9 kDa).
What kind of molecule is proteinase K?
Proteinase K is a subtilisin-related serine protease that hydrolyzes a variety of peptide bonds and is frequently used to cleanup enzymatic reactions or cell lysates.
What is the function of proteinase K in the DNA extraction process?
Proteinase K is used during DNA extraction to digest many contaminating proteins present. It also degrades nucleases that may be present in DNA extraction and protects the nucleic acids from nuclease attack.
Is proteinase K soluble in water?
As a general rule, proteinase K is stable and very active in buffers that con- tain denaturing reagents such as urea, sodium dodecyl sulfate (SDS), and guanidinium salts. Proteinase K is soluble at least up to 20 mg/ml in double-distilled water.
Is proteinase K Stable at room temperature?
Storage conditions and preparation of working solution Liquid Proteinase K can be stored at room temperature (18–25 °C) and is stable for at least one year.
What is proteinase K used for?
Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.
What is the work of proteinase K?
Proteinase K Product Information It cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples. It has been purified of RNase and DNase activities.
How is proteinase K used in DNA extraction?
During the extraction of DNA (or nucleic acids in general), there are many contaminating proteins present. These contaminants must be removed. Proteinase K, which is a broad spectrum serine protease, is used in many DNA extraction protocols to digest these contaminating proteins.
What is proteinase K dissolved in?
What is proteinase K solution?
Proteinase K Solution is a highly active serine protease that exhibits broad cleavage specificity. Isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation.
How long is proteinase K Stable for?
QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.
Does proteinase K work in water?
Proteinase K is very water soluble, and it can also be dissolved in Tris or PBS. When working with PBS, however, it can be a little tricky, which is possibly due to the pH (still within optimal range, but on the lower end of that range).
What is the concentration of proteinase K in DNA extraction?
The working concentration of Proteinase K is usually 50-400+ ug/mL. How much you will want to use depends on what tissue type you are working with. If you add 10uL of 20mg/mL Proteinase K stock to 990uL of extraction buffer, that would give you a working concentration of 200ug/mL, which is plenty for almost anything.
What is proteinase K used for in biology?
Proteinase K, Molecular Biology Grade. Proteinase K is a subtilisin-related serine protease that hydrolyzes a variety of peptide bonds and is frequently used to cleanup enzymatic reactions or cell lysates. Active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0.
When was proteinase K discovered?
The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album ). Proteinase K is able to digest hair ( keratin ), hence, the name “Proteinase K”.
How do you Digest proteinase K at 50 degrees?
Proteinase K digestion is routinely performed at 50°, and occasionally in the presence of EDTA in order to inhibit labile, Mg ++ -dependent nucleases. Proteinase K is prepared commonly as a 20 mg/ml stock solution in sterile water (stable for 1 year at −20°) or in a solution of 50 mM Tris, pH 8.0, 1 mM CaCl 2 (stable for months at 4°).
What is the cleavage site of Proteinase K?
Proteinase K is able to digest hair (keratin), hence, the name “Proteinase K”. The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups.