What is API 20E test strip used for?
This API-20E test strip (from bioMerieux, Inc.) is used to identify the enteric gram negative rods (although API makes a variety of other test strips for yeast, Staph, anaerobes, etc.) 20 separate test compartments are on the strip, all dehydrated. A bacterial suspension is used to rehydrate each of the wells.
What does API 20E stand for?
Analytical Profile Index
API (Analytical Profile Index) 20E is a biochemical panel for identification and differentiation of members of the family Enterobacteriaceae. It is hence a well-established method for manual microorganism identification to the species level.
What is API strips in bacterial identification?
API strips give accurate identifications based on extensive databases and are standardized, easy-to-use test systems. The kits include strips that contain up to 20 miniature biochemical tests. All are quick, safe and easy to perform.
What is API 10s strip?
The API® 10 S strip consists of 10 microtubes containing dehydrated substrates. These microtubes are inoculated with a bacterial suspension that reconstitutes the media. During incubation, metabolism produces color changes that are either spontaneous or revealed by the addition of reagents.
How do you read e test results?
Read MIC at the point where the ellipse intersects the scale. If a MIC value between two twofold dilutions is seen, always round up to the highest value. Read the MIC value at complete inhibition of all growth. If the intersect differs on either side of the strip, read the MIC as the greater value.
Who uses the API 20E system?
One of the API systems is specific for differentiating between members of the Gram negative bacterial Family Enterobacteriaceae and is called API-20E. The other API system is specific for Gram positive bacteria, including Staphylococcus species, Micrococcus, species, and related organisms, and is called API-Staph.
What is indole in microbiology?
The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to convert tryptophan into indole. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as “tryptophanase.”
What is API 50 CH test?
The API® 50 CH test is used to study bacterial growth on different carbohydrates (Table 1) and its derivatives (heterosides, polyalcohols, and uronic acids). The bacteria are resuspended in API 50 CHL medium or API® 50 CHB/E medium. The suspension is used to fill the tiny tubes (cupules) on the API strips.
What is API NH?
API NH® is a standardized system for the identification of Neisseria, Haemophilus (and related genera) and Moraxella catarrhalis (Branhamella catarrhalis), which uses miniaturized tests, as well as a specially adapted database.
What is the zone of inhibition?
The Zone of inhibition is a circular area around the spot of the antibiotic in which the bacteria colonies do not grow. The zone of inhibition can be used to measure the susceptibility of the bacteria to wards the antibiotic.
What does an E test show?
ETEST consists of a predefined gradient of antibiotic concentrations immobilized on a plastic strip and is used to determine the MIC of antibiotics and antifungal agents.
What indicates a positive indole test?
red color
A positive indole test is indicated by the formation of a red color in the reagent layer on top of the agar deep within seconds of adding the reagent. If a culture is indole negative, the reagent layer will remain yellow or be slightly cloudy.
Why is the indole test used?
The indole test screens for the ability of an organism to degrade the amino acid tryptophan and produce indole. It is used as part of the IMViC procedures, a battery of tests designed to distinguish among members of the family Enterobacteriaceae.
What is API 50 CH?
API 50 CHB/E Medium is intended for the identification of Bacillus and related genera, as well as Gram-negative rods belonging to the Enterobacteriaceae and Vibrionaceae families. It is a ready-to-use medium which allows the fermentation of the 49 carbohydrates on the API 50 CH strip to be studied. PRINCIPLE.
Is large zone of inhibition good?
Microban uses the Zone of Inhibition test to quickly assess the performance of specific antimicrobial agents. A very large zone of inhibition is not always interpreted as superior antimicrobial product protection, but rather can be an indicator of its instability and tendency to migrate.
Why do we use zone of inhibition?
How do you read e-test results?
What is API 20E used for?
API (Analytical Profile Index) 20E is a biochemical panel for identification and differentiation of members of the family Enterobacteriaceae. It is hence a well-established method for manual microorganism identification to the species level.
What are API identification products?
API identification products are test kits for identification of Gram positive and Gram negative bacteria and yeast. API strips give accurate identifications based on extensive databases and are standardized, easy-to-use test systems.
How do I use the api20e biochemical test strip?
Take the API20E Biochemical Test Strip which contains dehydrated bacterial media/bio-chemical reagents in 20 separate compartments. Using a pasteur pipette, fill up (up to the brim) the compartments with the bacterial suspension.