What is Caco-2 cell line?
Caco-2 [Caco2] are epithelial cells isolated from colon tissue derived from a 72-year-old, White, male with colorectal adenocarcinoma. This cell line is a suitable transfection host and has applications in cancer and toxicology research.
How do you culture Caco-2 cells?
The cells should be kept at 37 °C in a humidified atmosphere containing 5 % CO2. For propagation in culture flasks, Caco-2 cells are seeded in a concentration of 105 cells/cm2. Medium should be changed every 3 days. At 80 % confluence, typically after 4–5 days, the cells are split 1:10 before further cultivation.
What is permeability caco2?
Caco-2 permeability assay to investigate intestinal permeability. Cyprotex’s Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarised Caco-2 cell monolayers and from which the data generated can be used to predict in vivo absorption of drugs.
Why Caco-2 cells are used?
The Caco-2 cells isolated from human colorectal adenocarcinoma are widely used to screen for absorption rate of new compounds in the initial stage of drug discovery.
How do you thaw caco2 cells?
Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes.
What is Caco-2 permeability assay?
Cyprotex’s Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarised Caco-2 cell monolayers and from which the data generated can be used to predict in vivo absorption of drugs. The Caco-2 cell line is derived from a human colon carcinoma.
What is Caco-2 called?
Calcium–cobalt (1/2) | CaCo2 – PubChem.
What is Caco 2 permeability assay?
Why do you thaw cells quickly?
Cell freezing needs to occur at a slow, controlled cooling rate. In contrast, cell thawing works best when it is done quickly because the disappearance of ice around the cell does not have the same damaging effects as ice formation during cryopreservation.
How do you remove DMSO from a cell?
jeYes, you can dilute (1:10) the cells by cold PBS and centrifuge at 1200 rpm x 4 mins. This step will efficiently remove DMSO.
What is the difference between HEK293 and HEK293T cells?
The key difference between HEK293 and HEK293t is that HEK293 is an immortalized cell line derived from the embryonic human kidney that is transfected with sheared human adenovirus type 5DNA while HEK293t is a daughter cell line derived from HEK293 original cell line that is transfected with a plasmid vector carrying …
What does the 293 mean in HEK293?
human embryonic kidney cells
What Is HEK293? HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.
What is the Teer of Caco2 cells?
In our hands Caco2 cells grown on Costar Transwell inserts (polycarbonate, 12 mm diameter, 0.4 microm pore size) have a TEER of 600-1000 Ohmx cm2 by 14-21 days of culture. If cells are selected with vinblastin TEER can double, and easily reach 2000 Ohmxcm2.
Why use Caco-2 cells in intestinal absorption studies?
Caco-2 cells have been of great utility in intestinal absorption studies due to their exclusive ability to model human absorption characteristics and Caco-2 is the most commonly used intestinal permeability model.
What can we learn from Caco-2 monolayers of colon adenocarcinoma?
Caco-2 cell (human colon adenocarcinoma) monolayers have been widely used as an in vitro model to evaluate the absorption enhancement across the intestinal epithelium by assessing their TEER. The cadherins at AJs are a group of Ca 2+ -dependent adhesion molecules essential for the induction and maintenance of TJ assembly [ 149 ].
How long does it take to differentiate Caco2 cells from blank cells?
I seed Caco2 cells on 12 well permeable filters and grow them for 21 days for them to differentiate. I then measure TEER values of all wells including the blank (media only).