What is God Pod method?
The GOD-POD method is linear (up to 500 mg/dl), sensitive (detection limit 0.3 mg/dl), simple (requires 10 microlitre of sample to be incubated for 30 minutes with single reagent at room temperature) and requires simple instrumentation (the absorbance to be read between 505 nm to 550 nm).
What is the principle of glucose oxidase method?
Other uses of glucose oxidase include the removal of oxygen from food packaging and removal of D-glucose from egg white to prevent browning. PRINCIPLE: Glucose oxidase catalyses the oxidation of β-D-glucose to D-glucono- δ-lactone with the concurrent release of hydrogen peroxide (1).
What is the hexokinase method?
In the “hexokinase method,” glucose-6-phosphate formed from glucose and ATP by hexokinase (HK) is oxidized by NAD in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PD) to give NADH, which is quantitated spectrophotometrically at 340 nm (alternatively NADP+ may be used as the co-enzyme).
What does GOD PAP stand for?
“GOD-PAP“: enzymatic photometric test. Principle. Determination of glucose after enzymatic oxidation by glucose oxidase. The colorimetric indicator is quinoneimine, which is generated from 4-aminoantipyrine and phenol by hydrogen peroxide under the catalytic action of peroxidase (Trinder’s reaction) [3].
What is the normal 2 hour postprandial blood sugar?
Test results vary by age and are usually measured in milligrams per deciliter (mg/dL). Normal results for the two-hour postprandial test based on age are: For those who don’t have diabetes: less than 140 mg/dL. For those who have diabetes: less than 180 mg/dL.
Why is C peptide test done?
A C-peptide test is often used to help tell the difference between type 1 and type 2 diabetes. With type 1 diabetes, your pancreas makes little to no insulin, and little or no C-peptide. With type 2 diabetes, the body makes insulin, but doesn’t use it well. This can cause C-peptide levels to be higher than normal.
What is the purpose of the glucose oxidase test?
Glucose oxidase is widely used for the determination of free glucose in body fluids (medical testing), in vegetal raw material, and in the food industry. It also has many applications in biotechnologies, typically enzyme assays for biochemistry including biosensors in nanotechnologies.
What is the substrate for glucose oxidase?
A simple and inexpensive glucose oxidase substrate system was developed for enzyme immunoassay. This system is based on the formation of a coloured complex between polyvinyl alcohol or starch and iodine produced from iodide in the presence of hydrogen peroxide generated by the glucose oxidase reaction.
What is the purpose of hexokinase?
Hexokinase catalyzes the phosphorylation of glucose, the rate-limiting first step of glycolysis. Hexokinase II (HK-II) is a predominant isoform in insulin-sensitive tissues such as heart, skeletal muscle, and adipose tissues.
What is GOD method?
The GOD method divides each parameter into several classes in consonance to the weight of each parameter corresponding to the effect on groundwater vulnerability. Based on the results of the analysis, it is indicated that the area of PT. X is included in the category of negligible and moderate vulnerability.
What does GOD and POD mean?
GOD-POD (>> Co-occurring Abbreviation) Long Form: glucose oxidase-peroxidase.
Why does my blood sugar go up 3 hours after eating?
Why do people get blood sugar spikes after meals? When people eat a meal, especially when it contains carbohydrates, it is normal for them to have a temporary spike in their sugar level (often known as a post-prandial spike) before the insulin their body produces immediately starts working to lower the spike.
Which is a better method Folin Wu or Somogyi Nelson?
The new technique which has been compared to glucose oxidase method, Somogyi’s procedure and Folin-Wu method is easier and more accurate than the Somogyi’s original method, and gives blood glucose values almost identical with that obtained with glucose oxidase procedure.
How do you make Somogyi reagent?
(i) Dissolve 2.5 g anhydrous sodium carbonate, 2 g sodium bicarbonate, 2.5 g potassium sodium tartrate and 20 g anhydrous sodium sulphate in 80 mL water and make up to 100 mL.
What happens if C-peptide is high?
A high level of C-peptide can mean your body is making too much insulin. It may be a sign of one of the following conditions: Type 2 diabetes. Insulin resistance, a condition in which the body doesn’t respond the right way to insulin.
What C-peptide level is when diabetic?
C-peptide levels are associated with diabetes type and duration of disease. Specifically a c-peptide level of less than 0.2 nmol/l is associated with a diagnosis of type 1 diabetes mellitus (T1DM).