What is pUC18 used for?
Vectors pUC18 and pUC19 are small high-copy number plasmids that are widely used for cloning and manipulation of DNA fragments (9).
What is pUC18 DNA?
pUC18 is a commonly used plasmid cloning vector in E. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 enables successful cloning of large DNA fragments.
Where is pUC18 found?
pUC18 is isolated from E. coli strain HB101 by a standard plasmid purification procedure. Usage: pUC18 is a commonly used plasmid cloning vector in E. coli.
Is pUC18 ampicillin resistant?
The artificial plasmid pUC18 has been genetically engineered to include (1) a gene for antibiotic resistance to Ampicillin (amp R), and (2) a gene (and its promoter) for the enzyme beta-galactosidase (lacZ).
How many BP is pUC19?
2686 bp
Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length.
How big is the pUC18 plasmid?
Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation.
Which antibiotic resistant gene is present in the pUC18 vector *?
Ampicillin
The artificial plasmid pUC18 has been genetically engineered to include (1) a gene for antibiotic resistance to Ampicillin (amp R), and (2) a gene (and its promoter) for the enzyme beta-galactosidase (lacZ).
What type of vector is pUC18?
Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation.
What is pUC19 DNA?
Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.
What does pUC19 mean?
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation “pUC” is derived from the classical “p” prefix (denoting “plasmid”) and the abbreviation for the University of California, where early work on the plasmid series had been conducted.