What is resazurin in microbiology?
Overview. Resazurin (7-hydroxy-10-oxidophenoxazin-10-ium-3-one, sodium) is a blue fluorogenic dye used as a redox indicator in cell viability and proliferation assays for bacteria, yeast or mammalian cells.
What is the function of the resazurin?
Resazurin is used to measure the amount of aerobic respiration in streams. Since most aerobic respiration occurs in the stream bed, the conversion of resazurin to resorufin is also a measure of the amount of exchange between the water column and the stream bed.
What is the color of resazurin when it is reduced?
Active bacterial metabolism reduces resazurin irreversibly to a pink, fluorescent product called resorufin. As a result, a color change from blue to pink is representative of the presence of viable cells.
What color is resazurin when it is oxidized?
Resazurin assays are based on the reduction by living cells of the oxidized blue dye to a pink fluorescent resorufin product.
What is the resazurin test?
The resazurin reduction test is a rapid, inexpensive, and objective test for determining excessive microbial contamination in foods. This test has been used to assess microbial spoilage of ground beef and sliced raw and cooked meats.
How does the resazurin test work?
Resazurin gives milk a characteristic blue colour and the test is based on the ability of bacteria in the milk to reduce the blue dye.
What enzymes reduce resazurin?
Resazurin (blue) diffuses into cells where it is irreversibly reduced by diaphorase enzymes to a highly fluorescent (pink) compound, resorufin. In a subsequent, reversible reaction, resorufin may be further reduced to colorless, non-fluorescent hydroresorufin [26, 27].
What color is resazurin in the presence and absence of oxygen?
A particularly useful redox color indicator is resazurin in water. This redox indicator is colorless in an atmosphere devoid of oxygen but in an oxygen-containing atmosphere it has a pink color.
What is a resazurin assay?
The resazurin assay is commonly used to quantify the number of live cells in a sample, and to monitor cell viability / cytotoxicity. It is a fast, simple, accurate and homogeneous / no-wash high throughput assay that can be used to monitor cells for up to 24-48 hours.
What does a resazurin test measure?
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Can absorbance be used to monitor resazurin concentration?
The assay also can be monitored by absorbance although there is a slight loss of sensitivity [1]. Reduction of resazurin is believed to be accomplished by reductase or diaphorase-type enzymes from mitochondria and the cytosol [31].
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