Where do you swab for pertussis?
Optimal Specimen Collection for PCR Testing for Pertussis Specimens for PCR testing should be obtained by aspiration or swabbing the posterior nasopharynx. Throat swabs and anterior nasal swabs have unacceptably low rates of DNA recovery and should not be used for pertussis diagnosis.
What are the four methods used to identify Bordetella pertussis?
The diagnosis of pertussis is challenging and accurate laboratory identification of Bordetella infections remains problematic. Common laboratory diagnostic methods used for pertussis diagnosis include culture, direct-fluorescent-antibody testing (DFA), serology and polymerase chain reaction (PCR).
What is the best laboratory assessment of an infection of pertussis?
You should test with PCR from NP specimens taken at 0 to 3 weeks following cough onset. PCR may also provide accurate results for up to 4 weeks. After the fourth week of cough, the amount of bacterial DNA in the nasopharynx rapidly diminishes, which increases the risk of obtaining falsely-negative results.
Which of the following is the most suitable specimen for the isolation of Bordetella pertussis?
Nasopharyngeal secretions are the optimal specimens for isolating the pertussis bacterium and obtaining a lab confirmed diagnosis.
How do you get a nasopharyngeal swab?
Tilt patient’s head back 70 degrees. Gently and slowly insert a minitip swab with a flexible shaft through the nostril parallel to the palate until resistance is encountered. The distance is equivalent to that from the nostril to the ear of the patient, indicating contact with the nasopharynx.
How do you diagnose pertussis?
By taking a nose and throat swab, they can test for the Bordetella pertussis bacteria in culture or a more rapid polymerase chain reaction test. Your doctor may suggest a blood test to check your white blood cell count, which, if low, signals the presence of an infection.
How long does it take for pertussis swab results?
Results are reported in one to two weeks. A pertussis culture will allow the recovery of the bacteria in order to investigate potential outbreaks. Susceptibility testing can also be performed to determine which antibiotic to use for treatment.
How do doctors test for pertussis?
A nose or throat culture and test. Your doctor takes a swab or suction sample from the area where the nose and throat meet (nasopharynx). The sample is then checked for evidence of the presence of whooping cough bacteria.
What specimen is used for culturing Bordetella pertussis?
Patients with suspected pertussis should have a nasopharyngeal (NP) swab or aspirate obtained from the posterior nasopharynx to confirm the diagnosis. The optimal time to collect a culture specimen is during the first 2 weeks of illness because this is when viable bacteria are present in the nasopharynx.
Which media should be used to recover Bordetella pertussis from a nasopharyngeal specimen?
5 transport medium and using RL as the primary isolation medium are recommended for recovering B. pertussis from swab specimens.
What specimen of choice is used for Bordetella?
Nasopharyngeal swabs or aspirates are the specimens of choice for recovery of B.
How accurate is pertussis test?
Culture remains the gold standard for laboratory diagnosis of pertussis due to a specificity of essentially 100%. The CDC recommends that culture be performed during a suspected outbreak to confirm positive PCR results.
What specimen is used for culturing the organism of Bordetella pertussis?
Specimen should be a nasopharyngeal aspirate (best) or posterior nasopharyngeal swab. Throat and anterior nasal swabs have unacceptably low rates of recovery.
What Agar is used to isolate Bordetella pertussis?
Remel Bordet Gengou Agar
Remel Bordet Gengou Agar, when enriched with blood, is a solid medium recommended for use in qualitative procedures for the isolation of Bordetella pertussis and Bordetella parapertussis. Whooping cough is caused by B. pertussis and B.
How do I do a nasopharyngeal swab?
Insert the swab into the nostril, parallel to the palate. If you detect resistance to the passage of the swab, back off and try reinserting it at a different angle, closer to the floor of the nasal canal. The swab should reach a depth equal to the distance from the nostrils to the outer opening of the ear.
How do you do a nasal swab sample?
Do not touch the soft end with your hands or anything else. inch (1.5 cm) into your nose. swab, gently pressing against the inside of your nostril at least 4 times for a total of 15 seconds. Get as much nasal discharge as possible on the soft end of the swab.
How is Bordetella pertussis diagnosed?
What media is used for pertussis?
Stainer-Scholte synthetic broth medium and Bordet-Gengou blood agar both support growth of B. pertussis and are commonly used.
What is the specimen collection for pertussis?
Specimen Collection. Determining who has pertussis can be difficult. Whenever possible, clinicians should obtain a nasopharyngeal (NP) swab or aspirate from all persons with suspected cases. A properly obtained NP swab or aspirate is essential for optimal results (see Figures 1 & 2).
Can you use a nasal swab to test for pertussis?
Throat swabs and anterior nasal swabs have unacceptably low rates of DNA recovery and should not be used for pertussis diagnosis. The swab tips may be polyester (such as Dacron®), rayon, or nylon-flocked.
How do you test for pertussis with PCR?
Specimens for PCR testing should be obtained by aspiration or swabbing the posterior nasopharynx. Throat swabs and anterior nasal swabs have unacceptably low rates of DNA recovery and should not be used for pertussis diagnosis. The swab tips may be polyester (such as Dacron®), rayon, or nylon-flocked.
How do you collect a swab of the pernasal cavity?
Collection method 1 Seat the patient, looking upwards with the neck fully extended 2 Insert the pernasal swab through a nostril and advance along the floor of the nose until it reaches the nasopharynx. 3 It has been suggested that the swab be held against the posterior nasopharynx for up to 30 seconds or until the patient coughs.