What can calmodulin bind to?
A calmodulin-binding protein of 65 kDa, called 65-CMBP or p65, has been found in several secretory vesicles, such as synaptic, neurohypophyseal, chromaffin, platelets and pancreatic islet granules. This protein also binds to phospholipids with high affinity.
What is calmodulin-binding peptide?
The calmodulin-binding peptide (CBP) is a short 26-residue sequence derived from the C-terminus of skeletal muscle myosin light chain kinase that binds specifically to calmodulin (reviewed in Terpe, 2003).
How does calmodulin bind to calcium?
Calmodulin is a small dumbbell-shaped protein composed of two globular domains connected together by a flexible linker. Each end binds to two calcium ions. PDB entry 3cln , shown here, has all four sites filled with calcium ions and the linker has formed a long alpha helix separating the two calcium-binding domains.
What happens when calcium bind to calmodulin?
When calcium is bound to calmodulin a helix-loop-helix is formed along the backbone and a conformational change occurs. This conformational change, coupled with the flexibility of the protein due to the flexible connecting linker, allows calmodulin to interact with and bind to a wide variety of other proteins.
Which protein is closely related to calmodulin?
In place of troponin, smooth muscle cells contain a large amount of another regulatory protein calledcalmodulin (Figure 8-3). Although this protein is similar to troponin, it is different in the manner in which it initiates contraction. Calmodulin initiates contraction by activating the myosin cross-bridges.
What happens when calcium binds calmodulin?
How is calmodulin activated?
Activation. Calmodulin is activated by intracellular calcium. When calcium concentrations rise, the calcium ions are able to bind to calmodulin at a special motif called an EF hand domain. This configuration of amino acids is characteristic of calcium-binding proteins.
How does calmodulin bind calcium?
What happens when calcium binds to calmodulin?
What are calmodulin-binding proteins?
Calmodulin-binding proteins are, as their name implies, proteins which bind calmodulin. Calmodulin can bind to a variety of proteins through a two-step binding mechanism “conformational and mutually induced fit “, where typically two domains of calmodulin wrap around an emerging helical calmodulin binding domain from the target protein.
Does calmodulin bind to CBP?
Though calmodulin binds very strongly with CBP (nanomolar affinity), this interaction is dependent on calcium and the bound protein can be eluted in a single step using gentle buffers containing a calcium-chelating agent, such as EGTA.
What does calmodulin Sepharose 4B mean?
Calmodulin Sepharose 4B affinity resin for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.
What is 4b affinity resin used for?
Calmodulin Sepharose 4B affinity resin for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells. For single-step purification of native calmodulin-binding proteins