What are plat e cells?
The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.
What is a packaging cell line?
Vectors produced by the new packaging cell lines efficiently transduced G1/S arrested cells in vitro and terminally differentiated neurons in brain of immunocompetent rat. The new packaging cell line allows large-scale production of lentivirus vectors and therefore will facilitate human gene therapy efforts.
What are 293T cells?
The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. Age fetus Comments. This cell line is competent to replicate vectors carrying the SV40 region of replication.
What are Phoenix ECO cells?
Phoenix Ecotropic (ECO) cell line expresses ecotropic envelope protein. CD8 expression is a direct reflection of intracellular gag-pol and the stability of the producer cell population’s ability to produce gag-pol can be readily monitored by flow cytometry.
What is viral packaging cell line?
It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes.
What is the difference between 293 and 293T cells?
The 293T cells are derived from 293 cells but stably express the SV40 large T antigen which can bind to SV40 enhancers of expression vectors to increase protein production. 293T are also neomycin resistant due to the presence of a neomycin resistance cassete together with the SV40 Large T .
Why are HEK293T cells used?
HEK293 are frequently used for heterologous expression of cell membrane receptors and ion channels, and many studies have utilized this cell line to investigate the signalling pathways of G protein-coupled receptors (GPCRs) which are implicated in disease.
What is VSV G protein?
Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein. To study the mechanism of VSV G protein mediated membrane fusion, we set up a cell-cell fusion system in which cells are marked by different fluorescent proteins.
What does ampho stand for?
Phoenix Amphotropic (AMPHO) cell line expresses amphotropic envelope protein. CD8 expression is a direct reflection of intracellular gag-pol and the stability of the producer cell population’s ability to produce gag-pol can be readily monitored by flow cytometry.
How accurate is ATCC’s product sheet?
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only.
What does ATCC mean for living cultures?
For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product.