How do you prepare the root of a Monocot slide?
SLIDE PREPARATION OF STEM AND ROOT
- Take 2-3cm long pieces of the material.
- Hold the material between thumb and first finger of your left hand.
- Hold the razor in the right hand with edge of the blade facing you and handle at right angle to it.
- Dip the top of the material in water.
How do you identify the root of a monocot?
In monocot roots, xylem and phloem tissue bundles are arranged in a circular fashion around the central pith, which consists of ground tissue (parenchyma). Monocot roots have a larger number of vascular structures than dicot roots.
How do you prepare a microscope slide?
To prepare the slide:
- Place a drop of fluid in the center of the slide.
- Position sample on liquid, using tweezers.
- At an angle, place one side of the cover slip against the slide making contact with outer edge of the liquid drop.
- Lower the cover slowly, avoiding air bubbles.
- Remove excess water with the paper towel.
How would you distinguish monocots from dicots?
Monocots have flower parts in threes or multiples of threes as shown in the flowers to the left. Dicots have flower parts in multiples of fours or fives like the five-petaled dicot flower pictured to the right.
How would you differentiate between the stems of monocot and dicot plants?
The main difference between monocot stem and dicot stem is that monocot stem contains scattered vascular bundles across the stem whereas dicot stem contains vascular bundles arranged in the form of one or two rings. Monocot stem and dicot stem are the two types of stem structures in flowering plants.
What stain is used for cheek cells?
stain methylene blue
Certain stains are also used to stain specific cell structures or cell products. With cheek cells the stain methylene blue can be used.
How would you prepare a microscope slide?
Is Casparian strip present in monocot root?
The casparian strip is present in both monocot and dicot root. The casparian strip is the suberised, water-impermeable layer present in the endodermis.
How would you distinguish monocots from diverse?
How would you distinguish monocots from dicots?…Plant Kingdom.
| Dicots | Monocots |
|---|---|
| 1. Two cotyledon in their seed. | 1. One cotyledon in their seed. |
| 2. Generally have tap root system. | 2. Fibrous root is present. |
| 3. The leaf veins are reticulate in nature. | 3. The leaf veins are parallel in nature. |
What are two features that can be used to distinguish dicot stems from monocot stems?
Dicot stems have their vascular bundles in a ring arrangement. Monocot stems have most of their vascular bundles near the outside edge of the stem. The bundles are surrounded by large parenchyma in the cortex region. There is no pith region in monocots.
What features characterize a monocot stem?
Features of Monocotyledonous Stems:
- The vascular bundles are many.
- The stele is broken up into bundles.
- The endodermis is not found.
- The vascular bundles are collateral and closed.
- Leaf trace bundles are numerous.
- Each vascular bundle remains surrounded by a well developed sclerenchymatous sheath.
Why is methylene blue used on cheek cells?
Methylene blue is a stain that will be soaked up by your cells so that you can see them more easily. It will make the cell membrane, nucleus, and ribosomes darker than the rest of the cell. Without it your cells are mostly clear and difficult to see.
How do you prepare a cheek cell slide?
Preparing a cheek cell slide. Take a clean cotton swab and gently scrape the inside of your mouth. Smear the cotton swab on the centre of the microscope slide for 2 to 3 seconds. Add a drop of methylene blue solution and place a coverslip on top. Remove any excess solution by allowing a paper towel to touch one side of the coverslip.
How do you pick up cheek cells with a cotton bud?
The cotton bud will pick up cheek cells without damaging any tissue. Rub the side of the cotton bud with the cells on it onto the centre of a microscope slide. Put one drop of methylene blue stain on top of where the cells have been put on the slide. Carefully place one side of the coverslip into the methylene blue using a mounted needle.
How is cheek cell wet mount preparation performed?
Once the cells have been obtained, the following procedure is used for cheek cell wet mount preparation: any excess solution can be removed by touching one side of the slide with a paper towel or blotting paper.
How to use a cotton bud as a microscope?
Method 1 Set up the microscope using the lowest power objective lens. 2 Wipe one side of the cotton bud down the inside of your cheek. 3 Rub the side of the cotton bud with the cells on it onto the centre of a microscope slide. 4 Put one drop of methylene blue stain on top of where the cells have been put on the slide.