What is a flag vector?
FLAG® Tag Expression Vector Maps and 3xFLAG® Peptides Bacterial and mammalian expression vectors, or expression constructs, are circularized DNA transfection plasmids that are commonly used to overexpress recombinant proteins of interest.
What does the cross on Swedens flag mean?
The Nordic cross design traditionally represents Christianity. The design and colours of the Swedish flag are believed to have been inspired by the present coat of arms of Sweden of 1442, which is blue divided quarterly by a cross pattée of gold, and modelled on the Danish flag.
Was Sweden neutral in ww2?
Sweden maintained its policy of neutrality during World War II.
How do I make a cross in Inkscape?
in the Tool Box on the left of the Inkscape window (or use the keyboard shortcut F4) to select the Rectangle Tool….The cross consists of a horizontal bar and a vertical bar.
- Draw the horizontal bar.
- Draw the vertical bar.
- Merge the bars into a cross.
What is the V5 epitope?
The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. The V5 tag is generally used with all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence.
How long is V5 tag?
There are two sizes of the V5 tag ranging from 9–14 amino acids, although the longer tag is usually used.
What is a 3x FLAG tag?
3x Flag-tag is often used in tandem purification and protein purification. As mentioned above, 3x-Flag-tag is longer than 1x Flag-tag and has more charged amino acids. Negatively and positively charged amino acids potentially interfere more strongly with the POI and may influence protein expression.
Why do we use a flag tag?
Typically the Flag® tag is used for protein purification from mammalian expression systems or general immunostaining and immunoprecipitation assays.