What is EC medium?
EC Medium EC Medium, otherwise known as EC Broth, is used to detect the presence of coliform bacteria in drinking water, wastewater, and foods. Developed by Hajna and Perryl, it was designed for coliform bacteria, specifically E. coli.
What is brilliant green bile Broth?
Brilliant green bile (BGB) broth is a modification of MacConkey’s liquid medium for the isolation of Enterobacteriaceae and has been formulated to attain maximum recovery of bacteria of the coli-aerogenes group, while inhibiting most gram-positive organisms that might hinder the development of the bacteria sought.
Why is MacConkey broth used for E. coli?
This broth contains lactose which, when degraded, gives acid and gas, according to the definition indicating the presence of E. coli. The gas formed is collected in DURHAM tubes and acid production is detected by the indicator bromocresol purple, which turns yellow.
How do you make MacConkey broth?
Preparation Instructions Suspend 40.00 grams of MacConkey Broth in 1000 mls of distilled water. Heat to dissolve the medium completely. Distribute into tubes containing inverted Durham’s tubes and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.
How do you make tryptone Broth?
Tryptone Broth is used for the detection of indole production by coliforms. Suspend 15 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
What grows on Vrba?
Violet Red Bile Agar (VRBA) is a selective medium used to detect and enumerate lactose-fermenting coliform microorganisms….Composition of Violet Red Bile Agar (VRBA)
| Ingredients | Gms/liter |
|---|---|
| Lactose | 10.00 |
| Neutral red | 0.030 |
| Crystal violet | 0.002 |
| Agar | 15.00 |
Does E coli grow on MacConkey agar?
Streak plate of Escherichia coli and Serratia marcescens on MacConkey agar. Both microorganisms grow on this selective media because they are gram-negative non-fastidious rods.
Can E. coli grow on MacConkey agar?
What is Tryptone broth?
Tryptone broth is a nutrient broth consisting of 1% tryptone in distilled water. Tryptone is a stable product of protein digestion, and so is rich in amino acids… particularly tryptophan.
What is the difference between Tryptone and tryptophan?
Tryptophan is an amino acid that some bacteria can use for carbon and energy. Tryptone broth is rich in tryptophan, and thus serves as an excellent medium to use for testing for tryptophan digestion.
What is brilliant green bile agar used for?
Brilliant Green Bile Agar is used for isolating, differentiating and enumerating coliform bacteria. Noble and Tonney1 described Brilliant Green Bile Agar for determining the relative density of coliform bacteria in water and sewage. The medium is particularly useful in selectively isolating Salmonella spp.
Is Vrba selective?
Violet Red Bile Agar (VRBA) is a selective medium used to detect and enumerate lactose-fermenting coliform microorganisms.